Contamination of food-grade chicken eggs with pathogenic and conditionally pathogenic microorganisms in the process of their production, storage, and sale causes a high risk of toxicoinfections in humans. One of the most effective ways to reduce microbial load is to wash and disinfect the surface of eggshells with active chlorine-based products. The purpose of the study was to determine the quality of washing and disinfection of food-grade chicken eggs during storage in a chilled form. In the experiment, the quality and microbiological indicators of foodgrade chicken eggs were determined after washing and disinfection on the 1st, 60th, and 80th days of storage at a temperature of 4℃. The microbiological parameters of eggs were studied using MALDI TOF technology, and the quality was studied using digital ovoscopy. Washing and disinfection with a preparation based on active chlorine at a concentration of 0.5% freed eggshells from colonies of MAFAnM and mould fungi. Storage of washed and disinfected eggs in a chilled form until the 60th and 80th days contributed to an increase in the QMAFAnM by 2.45 lg CFU/cm2 and at 5.68 lg CFU/ cm2 accordingly. The quantity of mould fungi on the shell surface of washed and disinfected eggs on the 60th day of storage reached 4.37 lg CFU/cm2, and on the 80th day, their number was equal to the QMAFAnM. The quantity of MAFAnM and mould fungi in the yolks of food-grade eggs had a strong direct dependence on their quantity on the shell and on their shelf life in chilled form. Washing and disinfection of food-grade chicken eggs with a chlorine-containing preparation did not affect the loss of weight, albumen index, yolk colour, strength and thickness of shell, but reduced the HU index to 78.5-79.5 units, which, combined with microbiological indicators, allowed them to be stored in a chilled form up to 80 days. The results obtained should be factored in when choosing the product, washing and disinfection mode, and shelf life of food-grade eggs, considering the species composition of microorganisms characteristic of a particular poultry farm
shell; yolk; QMAFAnM; mould fungi; ovoscopy; microbial contamination
[1] Abdoli, B., Khoshtaghaza, M.H., Ghomi, H., Torshizi, M.A.K., Mehdizadeh, S.A., Pishkar, G., & Dunn, I.C. (2024). Cold atmospheric pressure air plasma jet disinfection of table eggs: Inactivation of Salmonella enterica, cuticle integrity and egg quality. International Journal of Food Microbiology, 410, article number 110474. doi: 10.1016/j.ijfoodmicro.2023.110474.
[2] Akarca, G., Istek, Ö., & Tomar, O. (2021). The effect of resin coating on the quality characteristics of chicken eggs during storage. Journal of Food Science, 86(4), 1243-1257. doi: 10.1111/17503841.15686.
[3] An, J.H., & Lee, H.S. (2023). Effect of the storage temperature on the quality of eggs inoculated with Salmonella enteritidis onto shell. Food Science and Biotechnology, 33(5), 1255-1260. doi: 10.1007/s10068-023-01402-1.
[4] Aygun, A. (2017). The eggshell microbial activity. In P.Y. Hester (Ed.), Egg innovations and strategies for improvements (pp. 135-144). New York: Academic Press. doi: 10.1016/B978-0-12800879-9.00013-5.
[5] Bermudez-Aguirre, D., & Niemira, B.A. (2023). A review on egg pasteurization and disinfection: Traditional and novel processing technologies. Comprehensive Reviews in Food Science and Food Safety, 22(2), 756-784. doi: 10.1111/1541-4337.13088.
[6] Borysevich, B., & Lisova, V. (2020). Microscopic changes in laying hens kidneys at the egg drop syndrome. Ukrainian Journal of Veterinary Sciences, 11(3), 46-55. doi: 10.31548/ujvs2020.03.005.
[7] Caner, C., & Yüceer, M. (2015). Efficacy of various protein-based coating on enhancing the shelf life of fresh eggs during storage. Poultry Science, 94(7), 1665-1677. doi: 10.3382/ps/pev102.
[8] Casale, R., Boattini, M., Bianco, G., Comini, S., Corcione, S., Garazzino, S., Silvestro, E., De Rosa, F.G., Cavallo, R., & Costa, C. (2023). Bloodstream infections by Pantoea species: Clinical and microbiological findings from a retrospective study, Italy, 2018-2023. Antibiotics, 12(12), article number 1723. doi: 10.3390/antibiotics12121723.
[9] Chan, H.Y., Hussin, A.S.M., Ahmad, N.H., Rukayadi, Y., & Farouk, A.E. (2021). Effectiveness of quaternary ammonium in reducing microbial load on eggs. Molecules, 26(17), article number 5259. doi: 10.3390/molecules26175259.
[10] Chan, S.H., Liau, S.H., Low, Y.J., Chng, K.R., Wu, Y., Chan, J.S.H., & Tan, L.K. (2023). A real-time PCR approach for rapid detection of viable Salmonella enteritidis in shell eggs. Microorganisms, 11(4), article number 844. doi: 10.3390/microorganisms11040844.
[11] Chousalkar, K.K., Khan, S., & McWhorter, A.R. (2021). Microbial quality, safety and storage of eggs. Current Opinion in Food Science, 38, 91-95, doi: 10.1016/j.cofs.2020.10.022.
[12] Di Franco, C., Beccari, E., Santini, T., Pisaneschi, G., & Tecce, G. (2002). Colony shape as a genetic trait in the pattern-forming Bacillus mycoides. BMC Microbiology, 2, article number 33. doi: 10.1186/1471-2180-2-33.
[13] Drabik, K., Batkowska, J., Próchniak, T., & Horecka, B. (2021). Citric acid as a factor limiting changes in the quality of table eggs during their storage. Poultry Science, 100(4), article number 100995. doi: 10.1016/j.psj.2021.01.018.
[14] DSTU 5028:2008. (2010). Chicken table eggs. Technical conditions. Retrieved from https://online.budstandart.com/ua/catalog/doc-page.html?id_doc=70433.
[15] Ezeh, P.A., Olayinka, B.O., Bolaji, R.O., Babangida, S.A., & Olowo-Okere, A. (2024). Phenotypic antibiotic susceptibility profile of clinical Enterobacteriaceae isolates from Kaduna State, northwest Nigeria. Access Microbiology, 6(6), article number 000747.v5. doi: 10.1099/acmi.0.000747.v5.
[16] Fikiin, K., Akterian, S., & Stankov, B. (2020). Do raw eggs need to be refrigerated along the food chain? Is the current EU regulation ensuring high-quality shell eggs for the European consumers? Trends in Food Science & Technology, 100, 359-362. doi: 10.1016/j.tifs.2020.04.003.
[17] Haralampidou, H., Ladomenou, F., Gkountoula, T., Mertzidis, P., & Giannousi, E. (2022). Pantoea agglomerans bacteremia: A rare case of bacteremia in an immunocompetent fouryear-old child. Cureus, 14(6), article number e26080. doi: 10.7759/cureus.26080.
[18] Hsu, S.C., Chen, H.L., Chou, C.F., Liu, W.C., & Wu, C.T. (2023). Characterization of microbial contamination of retail washed and unwashed shell eggs in Taiwan. Food Control, 149, article number 109718. doi: 10.1016/j.foodcont.2023.109718.
[19] Khan, S., McWhorter, A.R., Andrews, D.M., Underwood, G.J., Moore, R.J., Van, T.T.H., Gast, R.K., & Chousalkar, K.K. (2024). Dust sprinkling as an effective method for infecting layer chickens with wild-type Salmonella typhimurium and changes in host gut microbiota. Environmental Microbiology Reports, 16(3), article number e13265. doi: 10.1111/1758-2229.13265.
[20] Khatun, F., Khan, A.S., Ahmed, F., Rahman, M., & Rahman, S.R. (2022). Assessment of foodborne transmission of Salmonella enteritidis in hens and eggs in Bangladesh. Veterinary Medicine and Science, 8(5), 2032-2039. doi: 10.1002/vms3.874.
[21] Kho, C.J.Y., Lau, M.M.L., Chung, H.H., Chew, I.Y.Y., & Gan, H.M. (2023). Whole-genome sequencing of Pseudomonas koreensis isolated from diseased tor tambroides. Current Microbiology, 80(8), article number 255. doi: 10.1007/s00284-023-03354-5.
[22] Kukhtyn, M., Sverhun, Z., Horiuk, Y., Salata, V., Laiter-Moskaliuk, S., Mocherniuk, M., Kladnytska, L., & Horiuk, V. (2024). The influence of different methods of decontamination of microbial biofilms formed on eggshells. Potravinarstvo, 18, 666-682. doi: 10.5219/1981.
[23] Legros, J., Jan, S., Bonnassie, S., Gautier, M., Croguennec, T., Pezennec, S., Cochet, M.F., Nau, F., Andrews, S.C., & Baron, F. (2021). The role of ovotransferrin in egg-white antimicrobial activity: A review. Foods, 10(4), article number 823. doi: 10.3390/foods10040823.
[24] Lin, C.M., Herianto, S., Syu, S.M., Song, C.H., Chen, H.L., & Hou, C.Y. (2021). Applying a largescale device using non-thermal plasma for microbial decontamination on shell eggs and its effects on the sensory characteristics. LWT – Food Science and Technology, 142, article number 111067. doi: 10.1016/j.lwt.2021.111067.
[25] Lin, L., Liao, X., Li, C., Abdel-Samie, M.A., & Cui, H. (2020). Inhibitory effect of cold nitrogen plasma in Salmonella typhimurium biofilm and its application in poultry egg preservation. LWT – Food Science and Technology, 126, article number 109340. doi: 10.1016/j.lwt.2020.109340.
[26] Mahmoud, B.Y., Semida, D.A., Elnesr, S.S., Elwan, H., & El-Full, E.A. (2023). Approaches of egg decontamination for sustainable food safety. Sustainability, 15(1), article number 464. doi: 10.3390/su15010464.
[27] McWhorter, A.R., & Chousalkar, K.K. (2020). Salmonella on Australian cage egg farms: Observations from hatching to end of lay. Food Microbiology, 87, article number 103384. doi: 10.1016/j.fm.2019.103384.
[28] Medina-Gudiño, J., Rivera-Garcia, A., Santos-Ferro, L., Ramirez-Orejel, J.C., Agredano-Moreno, L.T., Jimenez-Garcia, L.F., Paez-Esquiliano, D., Martinez-Vidal, S., Andrade-Esquivel, E., & Cano-Buendia, J.A. (2020). Analysis of neutral electrolyzed water anti-bacterial activity on contaminated eggshells with Salmonella enterica or Escherichia coli. International Journal of Food Microbiology, 320, article number 108538. doi: 10.1016/j.ijfoodmicro.2020.108538.
[29] Obianwuna, U.E., Oleforuh-Okoleh, V.U., Wang, J., Zhang, H.J., Qi, G.H., Qiu, K., & Wu, S.G. (2022). Potential implications of natural antioxidants of plant origin on oxidative stability of chicken albumen during storage: A review. Antioxidants, 11(4), article number 630. doi: 10.3390/antiox11040630.
[30] Oliveira, G.D.S., McManus, C., Salgado, C.B., & dos Santos, V.M. (2022). Effects of sanitizers on microbiological control of hatching eggshells and poultry health during embryogenesis and early stages after hatching in the last decade. Animals, 12(20), article number 2826. doi: 10.3390/ani12202826.
[31] Oliveira, G.D.S., McManus, C., Vale, I.R.R., & dos Santos, V.M. (2024). Obtaining microbiologically safe hatching eggs from hatcheries: Using essential oils for integrated sanitization strategies in hatching eggs, poultry houses and poultry. Pathogens, 13(3), article number 260. doi: 10.3390/pathogens13030260.
[32] Petrovič, J., Mellen, M., Čmiková, N., Schwarzová, M., & Kačániová, M. (2024). Effects of laying hens housing system on eggs microbial contamination. Potravinarstvo, 18, 50-65. doi: 10.5219/1938.
[33] Shevchenko, L.V., Davydovych, V.A., Midyk, S.V., & Bezusa, O.O. (2021). Enrichment of chicken table eggs with lycopene and astaxanthin. Regulatory Mechanisms in Biosystems, 12(1), 9-13. doi: 10.15421/022102.
[34] Shevchenko, L.V., Davydovych, V.A., Ushkalov, V.O., Midyk, S.V., & Mykhalska, V.M. (2020). The effect of astaxanthin and lycopene on the content of fatty acids in chicken egg yolks. Regulatory Mechanisms in Biosystems, 11(4), 568-571. doi: 10.15421/022088.
[35] Singhal, N., Kumar, M., Kanaujia, P.K., & Virdi, J.S. (2015). MALDI-TOF mass spectrometry: An emerging technology for microbial identification and diagnosis. Frontiers in Microbiology, 6, article number 791. doi: 10.3389/fmicb.2015.00791.
[36] Sokołowicz, Z., Kačániová, M., Dykiel, M., Augustyńska-Prejsnar, A., & Topczewska, J. (2023). Influence of storage packaging type on the microbiological and sensory quality of free-range table eggs. Animals, 13(12), article number 1899. doi: 10.3390/ani13121899.
[37] Sokovnin, S. (2021). An electron beam technology of surface disinfection of the packed egg. Food and Bioproducts Processing, 127, 276-281. doi: 10.1016/j.fbp.2021.03.009.
[38] Stepień-Pyśniak, D., Marek, A., & Rzedzicki, J. (2009). Occurrence of bacteria of the genus Staphylococcus in table eggs descended from different sources. Polish Journal of Veterinary Sciences, 12(4), 481-484.
[39] Tajudeen, H., et al. (2024). Effect of dietary inclusion of Bacillus-based probiotics on performance, egg quality, and the faecal microbiota of laying hen. Animal Bioscience, 37(4), 689-696. doi: 10.5713/ab.23.0299.
[40] Tsai, M.Y., Shih, B.L., Liaw, R.B., Chen, W.T., Lee, T.Y., Hung, H.W., Hung, K.H., & Lin, Y.F. (2023). Effect of dietary supplementation of Bacillus subtilis TLRI 211-1 on laying performance, egg quality and blood characteristics of Leghorn layers. Animal Bioscience, 36(4), 609-618. doi: 10.5713/ab.22.0274.
[41] Wang, B., Wei, W., Aputexiakere, J., Li, Y., & Ma, H. (2022). Surface decontamination of whole eggs using pulsed light technology and shelf life study of combined pulsed light and vaseline coating during room temperature storage. Food Control, 137, article number 108411. doi: 10.1016/j.foodcont.2021.108411.
[42] Wilson, A., Chandry, P.S., Turner, M.S., Courtice, J.M., & Fegan, N. (2021). Comparison between cage and free-range egg production on microbial composition, diversity and the presence of Salmonella enterica. Food Microbiology, 97, article number 103754. doi: 10.1016/j.fm.2021.103754.
[43] Zeng, Q., Man, X., Huang, Z., Zhuang, L., Yang, H., & Sha, Y. (2023). Effects of rice blast biocontrol strain Pseudomonas alcaliphila Ej2 on the endophytic microbiome and proteome of rice under salt stress. Frontiers in Microbiology, 14, article number 1129614. doi: 10.3389/fmicb.2023.1129614.